Intana Bioscience offers interaction analysis of biomolecular interaction based on a single molecule sensitive spectroscopic approach called fluorescence cross-correlation spectroscopy (FCCS).
Advantages of FCCS
FCCS has many advantages, e.g.:
- It allows measurements in physiological environments (e.g. cell lysates, serum, intra-ocular fluids), circumventing purification steps
- It offers a comprehensive readout (KD, kon/off, diffusion coefficients, non-specific binding, aggregation, etc.)
- It is a generic technology (applicable to proteins, peptides, DNA/RNA, small molecules, etc.)
- It enables label-free determination of affinities and kinetics
- Its dynamic range is suitable for most physiologically-relevant interactions (pico- to micromolar range)
- It requires very little amounts (ng quantities)
|
